Aim:
The identification and differentiation of Candida albicans from other
yeasts by Germ Tube test.
Introduction :
Germ tube test is the
confirmatory test for Candida albicans. Germ tube formation was
first reported by Reynolds and Braude and hence the germ tube test is also
known as Reynolds-Braude Phenomenon. This is a rapid method for identifying and
differentiating C. albicans from other Candida
spp.
Buds and pseudo-hyphae can be distinguished from germ
tubes by the constricted attachment at the point of origin. Germ tubes don’t
show constriction at the point of origin.
Principle :
Germ tubes are short outgrowth, non-septate germinating
hyphae. They are ½ the width and 3 – 4 times the length of the cell from which
they arise. When cells of Candida are incubated in serum at 370C for
2-4 hours Candida albicans produce short, slender, tube like structures called
germ tubes. Formation of germ tubes is associated with increased synthesis of
protein and ribonucleic acid. Various media like fetal bovine serum may be used
as a substitute to human pooled serum.
um.
Requirements:
§ Culture: Suspected Candida
colonies from culture plate
§ Reagent: Serum (human,
sheep, fetal bovine)or other commercially produced media for germ tube testing
§ Others: Test tubes, loop or
wooden applicator, Pasteur pipettes, slides, cover slips
Procedure:
1.
Aliquot
0.5 ml (12 drops) of serum or media in a test tube.
2. Make
a light suspension of the suspect yeast colonies (by touching 1-2 large
colonies or 3-4 smaller colonies with a sterile wooden applicator stick or
loop) on serum.
3.
Incubate
the tube for 2-3 hours in a 35 – 37°C incubator.
4.
Place
a drop of the suspension on a slide using a Pasteur pipette and cover with a
coverslip.
5. Examine
the wet mount microscopically (at 40X) for production of germ tubes (long
tube-like projections extending out from the yeast cells).
Result and Interpretation:
A
short hyphal (filamentous) extension arising laterally from a yeast cell, with
no constriction at the point of origin. Germ tube is half the width and 3 to 4
times the length of the yeast cell and there is no presence of nucleus. Examples: Candida
albicans and Candida dubliniensis.
Ref:
https://microbiologyinfo.com/germ-tube-test-principle-procedure-results-interpretation-and-limitations/
https://microbeonline.com/germ-tube-test-candida-principle-procedure-results-interpretation/
Unfortunately there are a lot of AIDS/Herpes denials on Herbal cures still out there. I did research on them after I was tested HIV/Herpes positive I was so worried am I going to die soon. I continue my search again on herbal remedy for Std, then I found lots of testimony on how Dr Itua Herbal Medicine Cured HIV/Aids, Herpes Virus,Copd,HPV,Cancer, Hepatitis,Shingles, Diabetes,Epilepsy,Infertility, On websites sharing their testimonies, which made much more sense to me. All the authors pronounce Dr Itua As a man with Good Heart, I pick interest in their testimonies and I contact him about my situation then he gave me procedure how it works, I proceed after one week he courier his Herbal Medicine to me and instruct me on how to drink it for two weeks to cure. I receive His Herbal Medicine so I drank it for two weeks as I was told then after 2 days I go for a test I found out I was cured from HIV/Aids & Herpes Virus, I pay homage to him 2 months ago to his country to celebrate with him on his African festival which he told me it usually happens every year. I know there are lots of (HIV)/Aids Herpes Virus denials of Herbal Remedy movement the same few doctors and they represent a very small fraction of the community. I could have died because I refused Natural Herbs Cures for so long, but luckily, by the grace of God I am alive to tell my story. Contact Info...Whatsapp Number...+2348149277967,Email...drituaherbalcenter@gmail.com/My Instagram Username...avat5634 Just in case you need someone to talk with.
ReplyDelete