Yeast Cell Observation - Inverted Microscope

CDC GUIDELINES FOR INFECTION CONTROL

https://www.cdc.gov/infectioncontrol/guidelines/index.html 

ICMR GUIDELINES FOR INFECTION CONTROL

https://www.icmr.gov.in/pdf/covid/techdoc/EC_Guidance_COVID19_06052020.pdf 

Swine Flu- MMB I

 https://www.slideshare.net/sarangidipu/swine-flu-ppt-47316084

Antiviral Drugs - MMB I

https://www.slideshare.net/doctorrao/antiviral-drugs-15231072 

Antiviral Chemotherapy - MMBI

 https://www.slideshare.net/rajud521/antiviral-chemotherapy

HPLC PPT

https://docs.google.com/presentation/d/1J7nct0iX5HSMyeq9SV1mT3cJw265AdOBUN-Th1iokwg/edit?usp=sharing

Food Microbiology- Unit 4 & 5

 Food Borne Diseases :

1. https://docs.google.com/presentation/d/14YYpBvbW-mAYt2OmYBDiCwPqRKUhpyUKaAKRgay7E28/edit?usp=sharing

2. https://www.slideshare.net/HiwrHastear/food-poisoning-60301801

3.  https://www.slideshare.net/RaviKantAgrawal/foodborne-infections-and-intoxications

Parasitic Infections : 

4. https://www.slideshare.net/manjeetrathour1/parasiticfood-borne-diseases

Mycotoxins : 

5. https://docs.google.com/presentation/d/139D9NTttZOJQzESrZLlGrxKA7cw4IUJvmERd6y7jzkI/edit?usp=sharing

Investigation of Food borne Diseases: 

6. https://docs.google.com/presentation/d/1JAFoi_Z53MMMgZlM2HZxAZnXAiBRRLgY3VuXrLLkv1o/edit?usp=sharing

Biological and other Hazards : 

7.https://blog.smartsense.co/food-safety-education-month-hazards-prevention

Food Quality Control and Food Preservation

 Packaging, Traceability and Recall

Click the link: 

https://drive.google.com/file/d/1q23QgHsEhJds4UfEu6I0XWXN5CgHiHSE/view?usp=sharing 

HACCP Principles

https://drive.google.com/file/d/1ceD5By6jFPXUjs2RSh6fjSdNa7nwIu7g/view?usp=sharing

GMP (Good manufacturing Practices)

https://drive.google.com/file/d/1h6_Z8G5yL2lEyxOfy4SCyUkd4le3TS-e/view?usp=sharing

Food Safety policies: Environmental Policy, Glass Policy, Jewellary Policy and Environmental Policy

https://drive.google.com/file/d/1AMtpbz7WBIurCsEP8KK8krusC9upujCE/view?usp=sharing

Essential Commodities Act

https://drive.google.com/file/d/1HYyxb2RTM247K0Hsh8z_Z0FksOwJ5E3Z/view?usp=sharing

Emergency Preparedness in Food Industry 

https://drive.google.com/file/d/1Bn9PqjjEC73qC-7IdDaS3p5YKohxbIFt/view?usp=sharing

Codex Alimentarius 

https://drive.google.com/file/d/1ZtMHcRCbOu3bxBKmfHGI3B4Zd3_hAD_h/view?usp=sharing

FSSAI

https://drive.google.com/file/d/1xsF9f9mSfgcv5ZWLyW2gRtzJngIvZC2Y/view?usp=sharing

Food Adulteration

https://drive.google.com/file/d/1pgeaIjEqNh7wKXUgvkz_6cZhATRmKjhH/view?usp=sharing

BIS ( Bureau of Indian Standard)

https://drive.google.com/file/d/1rDpWWXztpZ7QqId5b5Qa0RrFacCgBb_2/view?usp=sharing

Food Safety Policies ( Environmental, Glass and Visitors policies)

https://docs.google.com/presentation/d/1YI1EzCBjCHwa3dzizf9TsGAE-6NwkQaZdEEJYJGo2cs/edit?usp=sharing

Laboratory Sampling Analysis

https://docs.google.com/presentation/d/1NlbjxfWSUNeysWPW0THlp6xC4abLNrzj1830xAea2-A/edit?usp=sharing

0.5 McFarland Standard

Use bacterial suspension with 0.5 McFarland turbidity (CLSI recommends) for antimicrobial testing of new antimicrobial agents (CLSI . The Number of bacterial cells present in the McFarland standard vary based on the cell size.
0.5 McFarland standard is equal to bacterial density of  1.5 x 10⁸  CFU/ml.

Uses :

For  Antimicrobial susceptibility testing, the inoculum does not contain a concentration of bacteria approximates the 0.5 McFarland turbidity standard, antimicrobial susceptibility test results will be affected. 

Resistant organism may appear susceptible if too less bacteria are used in the inoculum.

Procedure: 

McFarland Standard is mixing of 1% solution of barium chloride and 1% of sulfuric acid.

0.05 ml of 1 % barium chloride mixing with 9.95 ml of sulfuric acid to make 0.5 McFarland standard.

This standard can compare with bacterial suspension (saline) in another tube. If the bacterial suspension turbidity is less than McFarland standard add more bacteria can be added. If the bacterial suspension is more turbid than Macfarland standard add diluent. 

McFarlands standards and bacterial suspensions can be measured the OD at 600nm.

Mc Farland Standards

McFarland Std. No.	0.5	1	2	3	4
1% barium chloride (1ml)	0.05	0.1	0.2	0.3	0.4
1% Sulfuric acid  (1ml)	9.95	9.9	9.8	9.7	9.6
Cell density Approx. (1.5×108 CFU/ml)	1.5	3	6	9	12
Absorbance at 600nm	0.08 to 0.1	0.257	0.451	0.582	0.669

McFarland Std.