NOTIFICATIONS

Welcome

Tuesday, March 21, 2017

Industries, R&D Centers and Institutes in Coimbatore for Summer Trainings



Industries, R&D Centers and Institutes for Summer Internship

1.Biofertilizer Production, Plant  Phytochemical Analysis
Short tem Training on instruments   

T. STANES & COMPANY LIMITED
8/23-24, Race Course Road,
Coimbatore - 641 018.Tamilnadu
: +91 - 422 - 222 1514, 222 3515 - 3518.
Fax : +91 - 422 - 222 0432, 222 0857
Websitewww.tstanes.com



2. Plant Tissue Culture Training


     Sunglow Biotech
     No 12/14,Perur
    Vadavalli Road,
     Perur (P.O.)
     Coimbatore - 641 010.
     Tamilnadu, India








Office Phone

     91-422-6538546 



Mobile / Cell

     98422 - 33485

     92446 - 38689, 97877-33485


3. Tamil Nadu Agricultural University One day training on Organic Agriculture
Vermicomposting techniques
 Seed treatment techniques using biofertilizers and bioinoculants
 Panchagavya preparation
 Preparation of herbal insect repellants

The Professor and Head,
Department of Sustainable Organic Agriculture,
 TNAU, Coimbatore - 641 003.
 Telephone : 0422 6611206 / 0422 6611316

4. Production of Biofertilizers Training
 Professor and Head
Department of Agricultural Microbiology 
Tamil Nadu Agricultural University
Coimbatore - 641 003
Phone: 91-422-6611294
Fax: 91-422-2431672
Email: microbiology@tnau.ac.in

5. Mushroom Training at the Tamilnadu Agri University, Coimbatore
  1 day Training      5th of Every month (or)   next working day(if 5th is holiday)      Rs.150/head
  5 day Training      Need Based with pre payment                                                   Rs.2500/head

Hospitals and Laboratories to get Summer Training in Coimbatore.
1.     KMCH Ltd., Coimbatore

2.     PSG IMSR (Institute for Medical Science and Research)  –Peelamedu  Coimbatore  Contact Mail Id: microbiology@psgimsr.ac.in

3.     Kongunad Hospitals Pvt Ltd (Coimbatore)
No 116 & 117, 11th Street, Tatabad, Coimbatore, Tamil Nadu - 641012
Phone: 0422 249 4383, 0422 249 4363, 0422 249 4303, 0422 249 4373, 0422 431 6000, 0422 431 6431, 0422 249 8

       4.Royal care Hospitals, L&T Road ,Neelambur, Coimbatore - 641062             
  Telephone:0422 4040000 – 4040200, Email: royalcareneelambur@gmail.com
  Contact Department : Laboratory Medicine
4.     Microbiological  laboratories, RS Puram, Coimbatore.  Ph No: PH# 0422 4342018

5.     Bioline Laboratories, RS Puram, Coimbatore
No. 17-A, Civil Aerodrome Post,
Sitra,
Coimbatore - 641 014.
Phone No: 0244 4520099, 4270509.
Mail ID:
 info@biolinelab.com


6.     Spic Agro Biotech centre, (Plant Tissue Culture Training)
 No 399, Siruvani Road,
Pooluvapatti Post
Chittirai Chavadi
Alandurai,
Coimbatore - 641101,   Ph No: 0422-2650216, 2650192
     

7.      Aavin, Coimbatore

 Managing Director : Dr. Venkatachalam,B.V.Sc.,

Telephone Nos: 0422-2607951 / 2607961 /2607971

MD-Direct No : 0422-2607975

Fax – 0422- 2607975

email – aavincoimbatore@sify.com


8.      Moksha Foods & Beverages Pvt Ltd (Mr. Butler's - Food & Beverage)
 14/34 D,, Pappampatti Rd, Pallapalayam, Coimbatore, Tamil Nadu 641103
Call Us:
08049673687
Mobile:
  +91-8870005509
  +91-9566657073
Telephone:
  +91-422-6572450
  +91-422-2579355
9.     Senthil papain and Food Products Pvt Ltd.,
107 A, Senguptha st,
Ramnagar
Coimbatore- 09
Ph: 0422 – 4210993
Email :gm@papainindia.com

Thursday, March 16, 2017

Capsule staining



 Capsule Staining  (Negative Staining)

Aim:

To demonstrate the presence of capsule in microorganisms  .

Introduction:

A capsule is a gelatinous outer layer secreted by bacterial cell and that surrounds and adheres to the cell wall. Most capsules are composed of polysaccharides, but some are composed of glycoproteins and polypeptides. The capsule differs from the slime layer that most bacterial cells produce in that it is a thick, detectable, discrete layer outside the cell wall. Not all bacteria have the ability to form capsules even when the required genes are present. various environmental factors influence production of capsule.

Capsule facilitate the attachment of bacteria to surface, retard the loss of nutrients from the cell and help to scavenge or concentrate nutrients from the environments. Capsule staining is more difficult than other types of differential staining procedures because the capsular materials are water soluble and may be dislodges and removed with vigorous washing. Smears must not be heated because the resultant cell shrinkage may create a clear zone around the organism that is an artifact that can be mistaken for the capsule.  

          To visualize the bacterial capsules is by using negative staining Technique. During staining the non heat fixed bacterial smear with the acidic stains such as Nigrosin will not penetrate the bacterial cells (since both acidic stain and bacterial surface has negative charge). Instead the acidic stain deposits around the bacterial cells and create a dark back ground and the bacteria appear as unstained with a clear area around them, capsule.

Materials Required:

Bacterial Cultures  (Klebsiella pnemoniae, Bacillus Sp.,)

Glass Slides

Nigrosin

Inoculation loop. 

  

Procedure: 

1.   
1.      Placed a small drop of a Nigrosin, on the clean glass slide. 
Using sterile technique, added a loopful of bacterial culture to slide, smearing it in the dye.
2.      Used the other slide to drag the ink-cell mixture into a thin film along the first slide.
3.      The slide was allowed to air dry.
4.      The slide was air dried for few minutes and  examined microscopically (100X) for the presence of encapsulated cells as indicated by clear zones surrounding the cells.

 

 Result:

Capsule: Clear halos zone against dark background
No Capsule: No Clear halos zone